In Vitro Toothpaste Claim Services
Toothpaste Testing LaB: Science Behind Every Claim
Toothpaste testing lab for Whitening stain removal efficacy, Enamel remineralization claims, Sensitivity relief, RDA, REA, PCR, formulation, microbial claim studies.
TOOTHPASTE EFFICACY STUDY
Toothpaste Performance Studies Services
- 01 ABRASIVITY We valuate cleaning efficiency while ensuring safe enamel and dentin wear.
- 02 WHITENING EFFICACY We measure stain removal performance and improvement in tooth brightness.
- 03 REMINERALIZATION We assess mineral recovery and enamel strengthening after repeated treatment cycles.
- 04 SENSITIVITY RELIEF We evaluate dentinal tubule occlusion and long-term reduction in tooth sensitivity.
TOOTHPASTE ABRASIVITY TESTING
Abrasivity & Cleaning Performance
Relative Dentin Abrasivity (RDA)
We test your toothpaste for RDA/REA ISO 11609 to evaluate its abrasive effect on dentin while maintaining effective cleaning performance.
- Testing: Standardized RDA testing lab using reference abrasives to determine the abrasive potential of your toothpaste, with optional comparison against benchmark formulations.
- Data: Relative Dentin Abrasivity (RDA) value, dentin abrasivity assessment, and comparative performance data.
Pellicle Cleaning Ratio (PCR)
We test your toothpaste for its ability to remove stained pellicle from tooth surfaces and evaluate overall cleaning performance.
- Testing: Standardized Pellicle Cleaning Ratio (PCR) testing using controlled brushing protocols to assess cleaning efficiency.
- Data: Pellicle Cleaning Ratio (PCR) value, cleaning efficiency assessment, and comparative cleaning performance.
Enamel and Cavity Protection
Enamel Remineralization
We evaluate your toothpaste for its ability to restore minerals to demineralized enamel and strengthen tooth surfaces during a 15-day brushing study.
- Testing: Artificial enamel demineralization followed by a 15-day daily brushing treatment, with surface microhardness measurements, SEM imaging, and SEM-EDS mineral analysis at defined intervals.
- Data: Enamel hardness recovery (%), remineralization efficiency, mineral deposition profile, hardness recovery trend, and before-and-after SEM images.
Mineral Loss / Mineral Gain
We determine how effectively your toothpaste prevents mineral loss and promotes mineral recovery following repeated brushing.
- Testing: Controlled demineralization and remineralization study with quantitative elemental analysis to monitor changes in enamel mineral content.
- Data: Mineral loss/gain profile, calcium and phosphate recovery, and comparative mineral deposition results.
TOOTHPASTE Daily Brushing Treatment
Toothpaste Long Term Daily Exposure Testing Services
- Mechanical Brushing Simulation – We provide daily toothbrushing under controlled load, speed, and brushing cycles to evaluate toothpaste performance over time.
- Artificial Saliva Storage – We use specimens in a simulated oral environment between treatment cycles to replicate natural saliva exposure and mineral exchange.
- pH Cycling (Demineralization–Remineralization Model) – Our study model alternates between acidic and remineralizing conditions to mimic daily acid attacks and natural enamel recovery.
- Oral Environment Simulation – We recreate intraoral conditions including temperature, moisture, and treatment intervals to evaluate product performance under realistic laboratory conditions.
- Long-Term Treatment & Performance Evaluation – We monitor changes from Day 1 to Day 16, including whitening efficacy, remineralization, dentine tubule occlusion, and overall treatment durability using periodic measurements.
Efficacy Performance Testing
Stain Removal & Whitening Performance
Stain Removal Performance
We test your toothpaste for its ability to remove common extrinsic stains caused by tea, coffee, and other dietary staining agents.
- Testing: Standardized stain formation followed by controlled brushing and image-based color analysis throughout the study.
- Data: Percentage stain removal, before-and-after images, stain removal trend, and comparative performance data.
Whitening Efficacy
We evaluate how effectively your toothpaste improves tooth brightness and overall shade after repeated brushing.
- Testing: Standardized 15-day brushing study with objective color measurements and tooth shade evaluation before and after treatment.
- Data: Whitening improvement, color difference (ΔE), tooth shade improvement, and whitening performance trend.
Medical and Function Claims (For Cavities & Sensitivity)
Dentinal Tubule Occlusion
We test your toothpaste for its ability to reduce tooth sensitivity by sealing exposed dentinal tubules and forming a protective barrier against external stimuli. This study is designed to support sensitivity relief claims by evaluating both the effectiveness and durability of tubule occlusion.
- Testing: Human dentin specimens with exposed dentinal tubules are treated using a standardized 15-day brushing protocol. SEM imaging is performed before and after treatment to evaluate tubule occlusion, with an optional acid challenge to assess the durability of the protective layer.
- Data: A detailed report including percentage dentinal tubule occlusion, tubule diameter reduction, durability after acid challenge, before-and-after SEM images, and quantitative evidence supporting sensitivity relief claims.
BAsic and Safety claim studies
Gentle & Safe for Daily Use
We demonstrate that your toothpaste is suitable for repeated daily brushing while remaining gentle on both teeth and oral soft tissues.
- Gingival Cell Cytotoxicity (ISO 10993-5): We evaluate the biocompatibility of your toothpaste using gingival fibroblast cells to assess irritation potential and compatibility with oral soft tissues.
- Long-Term Brushing and Oral Simulation: We perform standardized repeated brushing studies to evaluate enamel wear, surface changes, and long-term formulation performance.
- pH Measurement: We determine the pH of your toothpaste to ensure the formulation remains within an appropriate range for oral use.
Ingredient & Label Claim Verification
We verify the presence or absence of key ingredients to support product labeling, regulatory compliance, and consumer confidence.
- Fluoride Content Analysis: We quantitatively measure fluoride concentration to confirm fluoride-free formulations or verify fluoride content where required.
- Sodium Lauryl Sulfate (SLS) Analysis: HPLC or LC-MS analysis is performed to confirm the absence or quantify the presence of Sodium Lauryl Sulfate in your formulation.
- Heavy Metal Analysis: We determine the concentration of regulated heavy metals to support compliance with applicable quality and safety requirements.
- Microbiological Quality Testing: We evaluate microbial quality to verify that the toothpaste is free from harmful microbial contamination and suitable for consumer use