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Dentin Tubule Penetration and Microleakage Study Lab

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Confocal Laser Microscope ( CLSM)  showing microleakage penetration
Nanowatts Dental In Vitro Lab Testing Services

Microleakage Dye Penetration Study : SEALING THE GAP

Dental restorative material testing lab for microleakage dentin tubule penetration study by SEM, confocal microscopy lab services for dental material research. 

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Microleakage Depth Penetration by ELectron Microscope

Sectioned tooth to be inspected under Scanning Electron Microscope ( SEM)

Detection of Microleakage using SEM

  • Cross Section Leakage Visualization: Scanning Electron Microscope SEM enables a clear view of microleakage dentin tubule penetration and widely used in dental restorative materials testing lab This allows for a precise measurement of penetration depth and the identification of areas where penetration may be inadequate. Visit our SEM Lab for more details.   
  • Analyzation of Surface Modifications: SEM is crucial in examining the impact of pre-treatment methods, such as etching or irrigation solutions, on the surface of dentin and causing sealer penetration. This allows optimization of clinical procedures to maximize the effectiveness of sealer.  
  • Enhanced Material Interaction Studies: The interaction between the sealer and the dentin wall, such as any chemical bonding or physical interlocking, can be examined in detail. This is especially important when evaluating new formulations or bioactive sealers designed to improve performance.  

SEM of tubular penetration  dentin root sealers

Visualizing Microleakage and Penetration Pathways

  • High-Resolution Imaging: Our Confocal microscopy lab services with SEM provides superior magnification and resolution, allowing researchers to visualize intricate details of the dental tubules and sealer. This capability ensures precise measurement of how deeply and uniformly the sealer penetrates the tubules, even at the nanoscale.  
  • Quantitative Data Assessment: In addition to qualitative imaging, SEM facilitates quantitative measurements of sealant penetration. Researchers can determine the depth of penetration, analyze variations across the dentin surface, and compare the performance of different sealers.  
  • Analyzing Dentin Surface Modifications: SEM is crucial in examining the impact of pre-treatment methods, such as etching or irrigation solutions, on the surface of dentin and the impact of sealer penetration. This allows optimization of clinical procedures to maximize the effectiveness of sealer.  
  • Versatility Across Materials: Whether evaluating traditional sealers, resin-based sealers, bioceramic sealers, or experimental formulations, SEM is adaptable for studying a wide range of materials and their behavior.
  • Visit our Scanning Electron Microscopy (SEM) lab to understand the technique. 

Tubular Depth Penetration by SEM lab demo report

SEM testing lab report for sealear tubule penetration on dentin sealers

Scanning Electron Microscopy (SEM) Lab Report on Microleakage Dentin Tubule Penetration Report

  1. Technical and executive summary of the testing objectives, methods used, key findings, and conclusions. 
  2. Graph plotting of zeta potential to give you a quick understanding of the overall results and their microleakage dentin tubule penetration report implications.


Micro microleakage dentin tubule penetration lab

Dental Lab

Dental RESTORATIVE Materials Testing By Stereo Microscope

Dye penetration test sample for stereo microscope

Dye Penetration test using Stereo Microscope

Preparation of Tooth Specimens 

  • Our dental restorative materials testing lab uses restored teeth are prepared by filling the canals with the sealer material provided under examination. 
  • Thermocycling is often employed to mimic the thermal stresses encountered in clinical conditions. 

  Dye Application 

  • The specimens are absorbed into a dye solution, such as methylene blue, basic fuchsin, or rhodamine B, for a while. Rhodamine B dye is often used in confocal microscopy lab services setup. 
  • The dye penetrates through gaps or inadequately sealed areas at the material-tissue interface.  

 Sectioning the Samples 

  • The longitudinal sectioning of the teeth is conducted using precision instruments, revealing the sealer-dentin interface. 

measurement of dye penetration depth using stereo microscope

Stereo Microscopy for Sealer Penetration Analysis

Evaluation of Sealing Efficiency 

  • Stereo microscope provides the ability to inspect microleakage dentin tubule penetration depth which are essential for successful endodontic treatment. 

Material Performance Comparison 

  • Dental restorative materials testing lab utilizes side-by-side comparison of various sealers, such as resin-based, bio ceramic, or zinc oxide sealers. 

Influence of Clinical Techniques 

  • Assess the impact of obturation techniques, such as cold lateral compaction or warm vertical condensation, on sealer performance. 

Quality Assurance in Research and Development 

  • Provides manufacturers with refining formulations for improved sealing efficiency and reduced microleakage

Visit our Dentin Deformation Lab

Mechanical Lab

Confocal MICROSCOPY Lab Services for Microleakage Study

Marginal sealing efficacy In Vitro study using Confocal Microscope ( CLSM)

Sealer Penetration By Confocal Microscopy CLSM

 Microleakage and Dye Penetration Studies: 

  • Our confocal microscopy lab services are used for dental sealers and adhesives to assess the sealing ability of restorative materials to prevent leakage at the tooth-restoration interface. 
  • Fissure sealants In Vitro study  to analyze the penetration depth of sealants in pits and fissures of teeth to improve preventive treatments. 
  • Orthodontic bonding and study adhesive penetration and bonding performance in orthodontic practices.  

 Dentin and enamel bonding studies:  

  • Our restorative materials testing lab uses advanced techniques in visualization of adhesive penetration into dentin tubules and enamel prisms.
  • Assessment of bonding strength by quantifying the resin-dentin interface to ensure optimal adhesion.  
  • Effect of surface treatments by assessing the impact of treatments like acid etching, air abrasion, and laser preparation on bonding efficacy.  


Dental Adhesive bonding dye penetration test using Confocal Microscope (CLSM)

Quantifying Resin-Dentin Interface by CLSM

  Dental Material Development and Quality Control: 

  • Polymerization depth tests and analysis of the polymerization depth and the curing efficiency of resin-based composites.  
  • Microleakage dentin tubule penetration combined with crack propagation to detect the microcracks in materials to enhance durability.  
  • Coating performance assessment of thickness and uniformity of coatings used in dental materials.  

  Effects of Aging and Degradation study:  

  • CLSM is used to study the long-term performance of dental materials in aging simulation to Evaluate material performance after simulated aging, including thermocycling and water storage.  
  • Dentin degradation analysis to investigate changes in material characteristics due to chemical or mechanical degradation.  
  • Fluoride penetration testing to determine the depth of fluoride incorporation into enamel for preventive treatment.  

Confocal Microscopy Lab Demo Report on Microleakage Sealers

Confocal Microscopy  CLSM Lab Report on Microleakage Dye  Penetration In Vitro Study

Confocal Laser Scanning Microscope (CLSM) Study for Depth Penetration In Vitro Study

  1. Technical and executive summary of the testing objectives, methods used, key findings, and conclusions from our confocal microscopy lab services. 
  2. Graph plotting designed to give you a quick understanding of the overall results and their implications.


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Nanowatts Technologies

80 ft Road, RMV 2 nd Stage, Bangalore 560094

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